Eagles captain Bryce Campbell said the Uruguay loss was tough to swallow.
"But that's sport, and that's life. You can lay down and go away, or you can find motivation to get back up on the horse and learn from it, and that's what we're going to do," he said.
Although losing their playoff game with Uruguay for the Americas 1 qualifying spot, the United States can still qualify for the Americas 2 berth that will be decided next year.
"We're really lucky that the All Blacks are coming over, and we get a chance to step back on the field. It'd be much harder if you had to step away and not play for another six months.
"One of our union's big concerns has been getting games against some of the top countries in the world. And now that we have it, we need to take the opportunity with both hands. The Kiwis play with an intensity and a speed that's like no other team in the world.
"It's really exciting that we're going to fact that and be able to challenge ourselves against it," he said.
The All Blacks and the USA Eagles will be playing in the inaugural Test for the 1874 Cup, which celebrates the year rugby was first played in the United States, in a collegiate match between McGill and Harvard Universities.
USA Rugby chief executive Ross Young said: "This is a special match for all of us within the USA rugby community, taking on one of the world's best in our nation's capital.
"With the Men's Eagles having last played on the east coast in 2017, we're thrilled to welcome eager fans to what is always a special experience facing the All Blacks," he said.
Coach and former Springboks assistant coach Gary Gold said: "It is exciting to round out our fall Test schedule with a fixture against the All Blacks.
"It's no secret this team is, and has been, one of the best in the world and the opportunity to go head-to-head at home is special. As the Rugby World Cup will be less than two years out, we look forward to heading into this Rugby World Cup calibre match."